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fadE [2018-02-13T06:22:54.000Z]
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fadE [2018-02-13T06:22:54.000Z]

acyl-CoA dehydrogenase
Locus
BSU32820
Isoelectric point
5.15
Molecular weight
65.16 kDa
Protein length
594 aa Sequence Blast
Gene length
1782 bp Sequence Blast
Function
fatty acid degradation
Product
acyl-CoA dehydrogenase
Essential
no
Synonyms
yusJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,367,040 → 3,368,824

    The protein

    Catalyzed reaction/ biological activity

  • Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF (according to Swiss-Prot)

    • Protein family

  • acyl-CoA dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • YngJ, MmgC, AcdA

    • Structure

  • 2Z1Q (from Thermus thermophilus hb8, 52% identity, 68% similarity)

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    fadN-fadA-fadE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • SdpR: activation, PubMed, in SdpR regulon
  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • FadN: induced by long-chain fatty acids (FadR) PubMed
  • expression of the operon is induced during biofilm formation PubMed
    • view in new tab

    Genes
    fadM-fadN-fadA-fadE
    Description
    PubMed

    Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • SdpR: activation, PubMed, in SdpR regulon

    • Regulation

  • fadM: induced by long-chain fatty acids (FadR) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A602 (yusJ::erm), available at the NBRP B. subtilis, Japan
  • BKE32820 (fadE::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
  • BKE32820 (ΔfadE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGC
  • BKK32820 (ΔfadE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGC

    • References

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